Modifying Enzymes
Gold Biotechnology Inc Proteinase K Solution 20 mg/ml 2 mL
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Cayman Chemical Coenzyme Q10 100mg
A cofactor in the electron transport chain; decreased levels due to genetic mutations in its synthesis enzymes lead to encephalopathy, cerebellar ataxia, infantile multisystemic form, nephropathy, and isolated myopathy
GENOVIS INC GlycINATOR LE, 2000 Units
GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that hydrolyzes all glycoforms present at the Fc-glycosylation site. The enzyme acts on the chitobiose core and leaves the core GlcNAc intact.
Takara Bio mRNA Interferase-MazF, 1000 units
MazF is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA at the 5' end of ACA sequences. This enzyme does not cleave double-stranded RNA, double-stranded DNA or single-stranded DNA.
Sigma Aldrich Fine Chemicals Biosciences Malonyl 13C3 coenzyme A li25MG
Malonyl 13C3 coenzyme A li25MG
New England Biolabs, Inc. RNase HII – 1250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
Research Products International Corp Macerozyme R-10, 1 Gram
Macerating enzyme from Rhizopus sp. Often used in combination with Cellulase (C32200). Optimum pH range 3.5 - 7.0.
New England Biolabs, Inc. β-N-Acetylhexosaminidase f - 500 units
b-N-Acetylhexosaminidasef is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylgalactosamine and glucosamine residues from oligosaccharides.
New England Biolabs, Inc. MspI Methyltransferase – 100 units
MspI Methyltransferase recognizes the same sequence as the HpaII Methyltransferase, but modifies the external cytosine residue (C5) of the sequence CCGG.
New England Biolabs, Inc. SP6 RNA Polymerase – 2000 units
Bacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions: 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the SP6 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. Lambda Protein Phosphatase – 100000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
Sigma Aldrich Fine Chemicals Biosciences Crotonoyl coenzyme A trili5MG
REAGENT Crotonoyl coenzyme A trili5MG
New England Biolabs, Inc. Bacteroides Heparinase III – 35 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
Sigma Aldrich Fine Chemicals Biosciences Heparinase I and III Blend
Heparinase is an inducible non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by flavobacterium heparinum and contains specific sequences of heparin.